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Ka hoʻoulu ʻana o ka follicular
Ma mua, pono ka makuahine e hana i ka lapaau hormonal hāʻawi ʻia e ka ʻinikini. ʻO ka pahuhopu o kēia: ka loaʻa ʻana o ka ulu ʻana o nā follicles lehulehu e ʻae ai i ka hōʻiliʻili ʻana o nā oocytes. ʻOi aku ka nui o nā manawa o ka hāpai ʻana. Mālama ʻia ka hoʻoulu ʻana (nānā) e kani ultrasound a nā hoʻokolohua hormonal. Ke oʻo nā follicles, hoʻoulu ʻia ka ovulation e kahi injection o nā hormones me ka hana LH: hCG.
Puncture o oocytes
Ma waena o 36 a me 40 mau hola ma hope o ka hoʻomaka ʻana o ka hoʻoulu ʻana, e ʻoki ʻia nā follicles ovarian i ka transvaginally. ʻO ka ʻoi aku ka pololei, ʻo ia ka wai i loko o kēlā me kēia follicle i loaʻa nā oocytes makua i hoʻomoʻa ʻia me ka nila. Hana ʻia ka puncture ma lalo o ka mana ultrasound a hana ʻia ma lalo o ka anesthesia kūloko a i ʻole, ʻoi aku ka pinepine, ma lalo o ka anesthesia āpau.
Ka hoomakaukau ana i na oocytes
A laila nānā ʻia ka wai follicular i loko o ka hale hana e ʻike ai i nā oocytes a hoʻokaʻawale iā lākou. Pono ʻoe e ʻike ʻaʻole pono nā follicles āpau i kahi oocyte a ʻo ia ʻAʻole hiki i nā oocytes a pau ke fertilizable.
Hoʻomākaukau ʻana i ka sperm
ʻO ka hōʻiliʻili o ka semen a me kāna hoʻomākaukau ʻana (ua holoi ʻia) hana maʻamau i ka lā o IVF i loko o ke keʻena. KAe koho ʻia ka sperm motile loa. No nā kumu like ʻole, hiki ke hōʻiliʻili maikaʻi ʻia ka sperm ma mua; no laila e hauʻoli lākou. I ka hihia o ka male male ʻole nui, pono paha e hui pū i nā oocytes a me ka spermatozoa (epididymal a i ʻole testicular punctures).
ʻO ka hoʻohua ʻana
Aia ma kahi kīʻaha moʻomeheu i loaʻa ka wai meaʻai ʻo ka pilina ma waena o ka spermatozoa a me nā oocytes. Hoʻokomo ʻia kēia i loko o kahi incubator ma 37 ° C. Pono ka mea hope e hoʻonāwaliwali i ka pūpū o ka oocyte i hiki i kekahi o lākou ke hoʻomomona iā ia.
ʻO ka fertilization a me ka ulu ʻana o ka embryonic
I ka lā aʻe, hiki iā mākou ke ʻike inā ua hoʻomomona ʻia kekahi oocytes. No ka ʻike pono i ka helu o nā embryos i loaʻa, pono e kali hou i 24 mau hola. Inā ua hoʻokō ʻia ka fertilization, hiki ke ʻike ʻia nā embryos me 2, 4, 6 a i ʻole 8 mau cell (e pili ana ka helu o nā cell i ka lā i ʻike ʻia ai). Hoʻololi ʻia nā embryos maʻamau ma hope o 2-3 mau lā ma hope o ke kī ʻana a i ʻole ka hau.
Hiki iā lākou ke hoʻololi iki i ka moʻomeheu lōʻihi a hiki i ka pae "blastocyst", ka pae hope o ka hoʻomohala ʻana ma mua o ka hāʻule ʻana.
Hoʻolilo Embryo
Hana ʻia kēia ʻano ʻeha a wikiwiki hoʻi i loko o ka hale hana IVF. Ke hoʻohana nei i kahi catheter lahilahi, kawaiho ʻia nā embryos i loko o ka ʻōpū. ʻO ka maʻamau, hoʻokahi a ʻelua mau embryo e hoʻoili ʻia a paʻa nā mea ʻē aʻe inā ʻae ka maikaʻi. Ma hope o kēia hana, kākoʻo ʻia ka pae luteal e ka lako progesterone i kēlā me kēia lā.
Nānā hāpai
Hoʻomaopopo ʻia ka hāpai ʻana e a hoʻopaʻa ʻia hormonal systematic a puni ka ʻumikumamākolu o ka lā ma hope o ka hoʻoili ʻana o ka embryo (ma IVF aia paha ke koko kumu ʻole e uhi ai i ka hoʻomaka ʻana o ka hāpai ʻana).
Pehea e pili ana i ka IVF me ICSI?
I ka wā o IVF me ICSI (intracytoplasmic sperm injection), i manaʻo nui ʻia no ka male ʻole o ke kāne, ʻokoʻa iki ke ʻano. Hoʻokahi wale nō sperm i koho ʻia. Hoʻokomo ʻia i loko o kahi oocyte a ma kahi kikoʻī. Ma hope o 19-20 mau hola, ʻike ʻia ka hele ʻana o ʻelua nuclei.